Nano-Enabled Zooremediation of Antibiotic Resistance Genes via Interfacial DNA Capture and Stimulated Nuclease Secretion
Nano-Enabled Zooremediation of Antibiotic Resistance Genes via Interfacial DNA Capture and Stimulated Nuclease Secretion
研究概述
将纳米零价铁与水丝蚓结合，利用铁相转变增强DNA磷酸骨架的界面捕集和片段化，再激活受体信号促进核酸酶分泌。实验微宇宙及废水中72小时使胞外耐药基因由10⁸降至10²拷贝/L，去除率99.9999%，并抑制水平基因转移。
谱图与表征信息
Figure 2. Phase transformation of nZVI and ARG distribution under different treatments. (a) XAFS linear-combination fits after 3 d; pie charts summarize phase fractions. Colors: yellow, Fe0; green, Fe2O3; brown, Fe3O4. (b) Mössbauer spectroscopy results showing the compositional changes of nZVI in different treatment groups after 3 d. (c) Confocal laser scanning microscopy images of ARG distribution in different treatment groups after 3 d; numbers indicate fluorescence intensities within the corresponding dashed-line regions. nZVI induces biochemical responses in worms to promote ARG degradation.